Figure 6.
Orai1 knockdown inhibits ICRAC in synthetic VSMCs and A7r5 cells. A–D) Silencing of Orai1 inhibits ICRAC recorded in DVF solutions in synthetic VSMCs (A, C). Similar results were obtained in A7r5 cells (B, D). STIM1 knockdown in A7r5 cells also inhibits ICRAC recorded in DVF solutions (B, D). E, F) Statistical analysis in synthetic VSMCs (E) and A7r5 cells (F), based on 3 to 4 independent experiments. G) Effect of 5 μM 2-APB on SOCE in wild-type synthetic VSMCs transfected with GFP (n=30) and in same cells transfected with CFP-Orai1 alone (n=16) or CFP-Orai1 + eYFP-STIM1 (n=28). Data are representative of 4 independent experiments. TG, thapsigargin.