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. Author manuscript; available in PMC: 2009 Jul 29.
Published in final edited form as: Radiat Res. 2009 May;171(5):513–520. doi: 10.1667/RR1658.1

FIG. 1.

FIG. 1

In situ immunofluorescence detection of p21Waf1 in control and low-fluence α-particle-exposed cell cultures. Density-inhibited AG1522 fibroblasts cultures were exposed to 0.13 cGy and incubated at 37°C for 3 h. Panel A: Extinction of p21Waf1 background fluorescence in sham-irradiated cultures (external control). Panel B: p21Waf1 expression in masked area of a partially irradiated cell culture (internal control). Panel C: Induction of p21Waf1 in cells in cultures exposed to 0.13 cGy (∼1 cell in 100 is traversed through the nucleus at this mean dose).