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. 2009 Aug 6;4(8):e6529. doi: 10.1371/journal.pone.0006529

Table 1. Compatibles Entry to Destination vector recombinations.

Entry vector type Compatible Destination vectors Incompatible Destination vectors
pENTR/pSM2 Series (non-GFP) pLenti X1 Series pLenti PGK Seriesb
pLenti X2 Series pLenti CMV Seriesb
pLenti CMV GFP pLenti CMV/TO Seriesb
pQCXI RNAi X2 Seriesa pQCXI CMV/TO Seriesb
pENTR/pSM2(CMV-GFP) pLenti X1 Series pLenti X2 Seriesc
pLenti CMV GFPd
pQCXI CMV/TO Seriesb
pQCXI RNAi X2 Seriesc
pENTR/pTER+ pENTR/pSUPER+ pLenti X1 Series pLenti PGK Seriesb
pLenti X2 Series pLenti CMV Seriesb
pLenti CMV GFP pLenti CMV/TO Seriesb
pQCXI RNAi X2 Series pQCXI CMV/TO Seriesb
pEF-ENTR Series pLenti X1 Series pLenti X2 Seriesc
pLenti CMV GFP pLenti PGK Seriesb
pLenti CMV Seriesb
pLenti CMV/TO Seriesb
pQCXI CMV/TO Seriesb
pQCXI RNAi X2 Seriesc
pENTR-Fusion Series pLenti PGK Series pLenti X1 Seriese
pLenti CMV Series pLenti X2 Seriesc,e
pLenti CMV/TO Series pLenti CMV GFPe
pQCXI CMV/TO Series pQCXI RNAi X2 Seriesc,e
a

Viral titers will be lower by ∼100 fold.

b

Probable promoter interference.

c

Insert would be too big for the 3′LTR.

d

Entry vector contains already a GFP.

e

No promoter to drive expression of the cDNA.