Table 1. Compatibles Entry to Destination vector recombinations.
Entry vector type | Compatible Destination vectors | Incompatible Destination vectors |
pENTR/pSM2 Series (non-GFP) | pLenti X1 Series | pLenti PGK Seriesb |
pLenti X2 Series | pLenti CMV Seriesb | |
pLenti CMV GFP | pLenti CMV/TO Seriesb | |
pQCXI RNAi X2 Seriesa | pQCXI CMV/TO Seriesb | |
pENTR/pSM2(CMV-GFP) | pLenti X1 Series | pLenti X2 Seriesc |
pLenti CMV GFPd | ||
pQCXI CMV/TO Seriesb | ||
pQCXI RNAi X2 Seriesc | ||
pENTR/pTER+ pENTR/pSUPER+ | pLenti X1 Series | pLenti PGK Seriesb |
pLenti X2 Series | pLenti CMV Seriesb | |
pLenti CMV GFP | pLenti CMV/TO Seriesb | |
pQCXI RNAi X2 Series | pQCXI CMV/TO Seriesb | |
pEF-ENTR Series | pLenti X1 Series | pLenti X2 Seriesc |
pLenti CMV GFP | pLenti PGK Seriesb | |
pLenti CMV Seriesb | ||
pLenti CMV/TO Seriesb | ||
pQCXI CMV/TO Seriesb | ||
pQCXI RNAi X2 Seriesc | ||
pENTR-Fusion Series | pLenti PGK Series | pLenti X1 Seriese |
pLenti CMV Series | pLenti X2 Seriesc,e | |
pLenti CMV/TO Series | pLenti CMV GFPe | |
pQCXI CMV/TO Series | pQCXI RNAi X2 Seriesc,e |
Viral titers will be lower by ∼100 fold.
Probable promoter interference.
Insert would be too big for the 3′LTR.
Entry vector contains already a GFP.
No promoter to drive expression of the cDNA.