Table 5.
Identification of rat plasma fibrinopeptides A and B using a nanoChip-LC/MS/MS instrument and the NCBInr database.
| ID | AC | Peptide mass [M] | Observed ion [M+nH]n+ | Charge | Ion score | Peptide sequence |
|---|---|---|---|---|---|---|
| Fibrinopeptide A | 650771P | 1105,56 | 553,79 | 2+ | 38 | EFIEAGGDIR |
| 1192,59 | 597,30 | 2+ | 62 | SEFIEAGGDIR | ||
| 1293,64 | 647,83 | 2+ | 73 | TSEFIEAGGDIR | ||
| 1394,69 | 698,35 | 2+ | 96 | TTSEFIEAGGDIR | ||
| 1451,72 | 726,87 | 2+ | 81 | GTTSEFIEAGGDIR | ||
| 1552,76 | 777,39 | 2+ | 103 | TGTTSEFIEAGGDIR | ||
| 1667,83 | 834,92 | 2+ | 108 | DTGTTSEFIEAGGDIR | ||
| 1738,86 | 870,44 | 2+ | 144 | ADTGTTSEFIEAGGDIR | ||
| 1738,88 | 580,63 | 3+ | 23 | ADTGTTSEFIEAGGDIR | ||
| 1582,59 | 792,30 | 2+ | 56 | ADTGTTSEFIEAGGDI | ||
| Fibrinopeptide B | 650771AE | 1061,54 | 531,78 | 2+ | 20 | DSDKVDLSIA |
| 1079,54 | 540,78 | 2+ | 37 | TTDSDKVDLS | ||
| 1150,57 | 576,29 | 2+ | 40 | ATTDSDKVDLS | ||
| 1162,58 | 582,30 | 2+ | 46 | TDSDKVDLSIA | ||
| 1318,66 | 440,56 | 3+ | 35 | TDSDKVDLSIAR | ||
| 1318,66 | 660,34 | 2+ | 46 | TDSDKVDLSIAR | ||
| 1334,66 | 668,34 | 2+ | 23 | ATTDSDKVDLSIA | ||
| 1419,70 | 474,24 | 3+ | 50 | TTDSDKVDLSIAR | ||
| 1419,70 | 710,86 | 2+ | 101 | TTDSDKVDLSIAR | ||
| 1490,74 | 746,38 | 2+ | 105 | ATTDSDKVDLSIAR |
Peptide sequences were observed in MASCOT results (ion scores are given in the table), and confirmed by de novo sequencing (in bold) using the PEAKS Studio 3.0 software (Bioinformatics Solutions Inc.). Blast E values were commonly ≤1 (≤0.2 for 75% of the searches).