Table 1.
Highly expressed genes with membrane-bound gene products up-regulated in cervical dysplasia stages CINI and CINIII. Up-regulated gene expression, from normal, ≥2-fold.
| Tag sequence | Number of tags in disease libraries* | Number of tags in normal libraries | Gene | Fold increase from normal | RefSeq accession number |
|---|---|---|---|---|---|
|
CINIII | |||||
| GACCCAAGATAAAAGAA | 704 | 16 | PIGR | 22.4 | NM_002644 |
| ATCCCCCTGGGCATCGG | 52 | 2 | SLC39A3 | 13.2 | NM_144564 |
| ACTCAGACCAGGTCCCA | 94 | 4 | STRA6 | 11.4 | NM_022369 |
| ACACAGTATTCGCTCTT | 44 | 2 | ITR | 11.2 | NM_180989 |
| TTACTTCCCCACCCCTA | 84 | 4 | FADS2 | 10.7 | NM_004265 |
| GGAACTGTGAAGAGGCA | 230 | 12 | TSPAN1 | 9.7 | NM_005727 |
| GCACCTGTCGCCCAGTG | 36 | 2 | ANPEP | 9.2 | NM_001150 |
| CCTGATCTGCGGTGTCC | 308 | 18 | MUC16 | 8.7 | NM_024690 |
| CGTTTTCTGATAACTCA | 68 | 4 | PTP4A2 | 8.6 | XM_001132367 |
| CAAATAAATTATGCGAT | 98 | 6 | TMPRSS2 | 8.3 | NM_005656 |
| TGCTCCTACCCTGCTCT | 2022 | 190 | FCGBP | 5.4 | XM_001131379 |
| GCAGTGCCACTCAAGAA | 726 | 68 | SRD5A2L | 5.4 | NM_024592 |
| CCTGGGAAGTGTTGTGG | 730 | 98 | MUC1 | 3.8 | NM_001044391 |
| AATATTTATATTGTATG | 1880 | 400 | CEACAM5 | 2.4 | NM_004363 |
| GTTCACATTAGAATAAA | 3424 | 762 | CD74 | 2.3 | NM_001025158 |
| GGGCATCTCTTGTGTAC | 1602 | 350 | HLA-DRA | 2.3 | NM_019111 |
| TGCTGCCTGTTGTTATG | 826 | 192 | BST2 | 2.2 | NM_004335 |
| CTGACCTGTGTTTCCTC | 596 | 144 | HLA-B | 2.1 | NM_005514 |
| GCAGGGCCTCATCTCAC | 1950 | 484 | FXYD3 | 2.0 | NM_005971 |
|
CINI | |||||
| GCACCTGTCGCCCAGTG | 42 | 2 | ANPEP | 36.5 | NM_001150 |
| ATGTTAATAAAATAGGC | 66 | 4 | GPC4 | 28.7 | NM_001448 |
| ATAAATGATTAGACTAC | 32 | 2 | CLIC6 | 27.8 | NM_053277 |
| GGCCAAGAACTTTCACT | 28 | 2 | C14orf101 | 24.3 | NM_017799 |
| GACCCAAGATAAAAGAA | 262 | 16 | PIGR | 23.0 | NM_002644 |
| TAGGTCAGGACCTTGGC | 26 | 2 | PTP4A3 | 22.6 | NM_032611 |
| GTATATAACTCTTAAAG | 24 | 2 | LOC644410** | 20.8 | XM_001133198 |
| CCAGCTGCCTGGAGGAG | 22 | 2 | MGC45438 | 19.1 | NM_152459 |
| ATAAAAATTAGGGGGAT | 22 | 2 | SLC30A1 | 19.1 | NM_021194 |
| TGAGCTACCCCAGAGTC | 20 | 2 | FER1L4 | 17.4 | NR_001442 |
| TACATCAGTAAAGAGTT | 374 | 42 | GAS1 | 12.5 | NM_002048 |
| CATCACGGATCAATAGA | 330 | 64 | IL1R1 | 7.2 | NM_000877 |
| CCTGGGAAGTGTTGTGG | 334 | 98 | MUC1 | 4.1 | NM_001044391 |
| TGCAGATTGCAGTTCTG | 366 | 132 | PROM1 | 3.9 | NM_006017 |
| CACTTCAAGGGCAGCCT | 238 | 102 | LY6E | 3.3 | NM_002346 |
| GTTCACATTAGAATAAA | 1660 | 762 | CD74 | 3.1 | NM_001025158 |
| GGGCATCTCTTGTGTAC | 734 | 350 | HLA-DRA | 3.0 | NM_019111 |
| TGCTCCTACCCTGCTCT | 402 | 190 | FCGBP | 3.0 | XM_001131379 |
| CTGACCTGTGTTTCCTC | 256 | 144 | HLA-B | 2.5 | NM_005514 |
*
GEO Series Accession Numbers GSE6252. 1,101,702 total longSAGE tags in four normal libraries, GEO Alias N3, N1, N2, N4; 2,165,777 total longSAGE tags in six CINIII libraries, GEO Alias C1, C3, C2, C4, C5, C6; 785,642 total longSAGE tags in three CINI libraries, GEO Alias M1, M2, M3.
**
Mapping unable to be confirmed through ENSEMBL or BLAT.