Table 1.
Digestion of catestatin-region synthetic peptide CgA360–380 by CTSL
| Mass (m/z) submitted | Mass (m/z) matched | Sequence |
|---|---|---|
| 840.4344 | 840.4475 | _ARAYGFR_G |
| 845.4232 | 845.4377 | R_RGWRPSS_ |
| 880.5095 | 880.5112 | F_RGPGPQLR_R or R_GPGPQLRR_G or G_PGPQLRRG_W |
| 1027.5590 | 1027.5796 | G_FRGPGPQLR_R |
| 1183.6744 | 1183.6807 | G_FRGPGPQLRR_G |
| 1545.8408 | 1545.8397 | _ARAYGFRGPGPQLR_R |
| 1550.8342 | 1550.8299 | R_GPGPQLRRGWRPSS_ |
| 1853.9360 | 1853.9994 | G_FRGPGPQLRRGWRPSS_ |
Catestatin region synthetic peptide was digested with CTSL for 15 min at 30 C, and the reaction products were analyzed by MALDI-TOF. Possible fragments generated in the catestatin region are listed in the table. CgA360–380 was 10 μm/reaction, and CTSL was 0.015 μm. hCgA360–380 synthetic peptide: ARAYGFRGPGPQLRRGWRPSS.