Table 1.
Primers for qPCR
| Gene | Accession no. | Sequence (5′–3′) | Region amplified | Referencea |
|---|---|---|---|---|
| Fshr | NM_199237.1 | Forward, ACGCCATTTTCACCAAGAAC | 1944–2022 | |
| Reverse, TGGGCTTGCATTTCATAACA | ||||
| Usf1 | NM_031777 | Forward, AAGTCAGAGGCTCCCAGGA | 693–761 | (42) |
| Reverse, CGGCGCTCCACTTCGTTAT | ||||
| Usf2 | NM_031139 | Forward, AGACCAACCAGCGTATGCAG | 1386–1473 | (42) |
| Reverse, GCTCCTCGGATCTGCTGCCT | ||||
| Ppia | M19533.1 | Forward, atggtcaaccccaccgtgt | 43–143 | |
| Reverse, tctgctgtctttggaactttgtct |
a
Primers without a reference were designed for use in this study with the aid of Primer Express software (version 3.0; Applied Biosystems).