Figure 7.
7F0 → 5D0 excitation spectra (5D0 → 7F2 emission) of solutions of 25 µM Eu2(1) titrated with HP1 DNA at pH 7.0, 20 mM HEPES, 50 mM NaNO3 (top). Data are fit to eq. 3 with a Kd of 2.5 µM and n of 8.2 (bottom).
7F0 → 5D0 excitation spectra (5D0 → 7F2 emission) of solutions of 25 µM Eu2(1) titrated with HP1 DNA at pH 7.0, 20 mM HEPES, 50 mM NaNO3 (top). Data are fit to eq. 3 with a Kd of 2.5 µM and n of 8.2 (bottom).