Figure 3.
Examination of the influence of culture vessel on GFP yield. Shake flask cultures were set-up with a working volume of 20% total vessel volume, the M24 with 60% and the bioreactor with 43%. Vented cultures had a 0.2 μm integral filter as a closure. Non-vented cultures had a foil closure. Culture conditions were 30°C, pH 6 and either 250 rpm in the shake flasks, or DO 30% in the M24 and the 7 L bioreactor. The model predicts a specific yield of 219 ng mL-1 OD595-1. Total yields for each condition are given.