Table 1.
Specification of the input factors and measurable outputs for the model building experiments.
| INPUT FACTORS (controlled on-line) | MEASURABLE OUTPUT (measured offline) | ||||||
| T (°C) | pH | DO (%) | OD595 | RFU (mL-1) | Specific RFU (mL-1 OD595-1) |
Specific yield (ng mL-1 OD595-1) | SD; n = 3 (ng mL-1 OD595-1) |
| 19 | 6 | 60 | 20.3 | 8651 | 426.2 | 127.9 | 3.2 |
| 19 | 8 | 60 | 0.8 | 1015 | 1268.8 | 380.6 | 3.9 |
| 19 | 7 | 30 | 13.1 | 10984 | 838.5 | 251.6 | 1.3 |
| 19 | 7 | 90 | 12.4 | 9259 | 746.7 | 224.0 | 2.1 |
| 24 | 6 | 30 | 24.4 | 8061 | 330.4 | 99.1 | 1.6 |
| 24 | 6 | 90 | 16.2 | 11951 | 737.7 | 221.3 | 5.6 |
| 24 | 8 | 30 | 4.7 | 1564 | 332.8 | 99.8 | 1.1 |
| 24 | 8 | 90 | 1.3 | 1954 | 1503.1 | 450.9 | 1.3 |
| 24 | 7 | 60 | 17.6 | 21382 | 1214.9 | 364.5 | 10.1 |
| 29 | 7 | 30 | 24.8 | 25392 | 1023.9 | 307.2 | 0.2 |
| 29 | 8 | 60 | 4.4 | 1413 | 321.1 | 96.3 | 1.5 |
| 29 | 6 | 60 | 21.7 | 10349 | 476.9 | 143.1 | 0.3 |
| 29 | 7 | 90 | 15.1 | 17495 | 1158.6 | 347.6 | 3.5 |
The input factors were temperature (T), pH and % dissolved oxygen (DO). Relative fluorescent units (RFU) and the optical density at 595 nm (OD595) were measured in triplicate 48 h post induction. The mean values are reported for 1 mL of culture. The standard deviation (SD; n = 3) is given for the specific yield of the M24 culture, where the conversion factor from RFU to ng was determined by generating a standard curve as described in the Methods section. All experiments were performed in batch in the M24 in BMMY medium.