Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Conditions of the bioreactor adaptation phase under optimal DoE conditions.
| Time (min) | T (°C) | pH | DO (%) |
| 0 | 30 | 6 | 30 |
| 15 | 28 | 6.4 | 45 |
| 30 | 25 | 6.8 | 60 |
| 45 | 23 | 7.2 | 75 |
| 60 | 21.5 | 7.6 | 90 |
| 75 | 21.5 | 7.6 | 90 |
The input factors, T (°C), pH and DO (%) were adjusted every 15 min over the course of 1 h via an ADI1030 controller. Once optimal DoE conditions (21.5°C, pH 7.6, 90% DO) were reached, a further 15 min stabilisation period was applied prior to fed-batch induction.
