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Inhibition of ERK-1/2 results in decreased nitric oxide levels. RBMM were pre-treated with U0126 (1 μM) or vehicle (MeOH) for 30 min prior to infection as described in Figure 1. Cell supernatants were analyzed for nitric oxide production after 24 hr. *p < 0.001 for BCG vs BCG+U, BCG+SP-A vs BCG+SP-A+U; n = 3.
