Table 3.
Activities of the enzymes used in hydrolysis
| Enzyme | Enzyme activity | ||||||
| FPA (FPU/g) | β-glucosidase (IU/g) | Xylanase (IU/g) | β-xylosidase (IU/g) | Endoglucanase (IU/g) | Mannanase (IU/g) | β-mannosidase (IU/g) | |
| Celluclast 1.5 L | 56.2 | 30.8 | 750 | 63.6 | 13087 | 122 | ~0.08 |
| Novozym 188 | n.d. | 502 | 34.7 | 6.7 | 296 | 17.9 | 5.4 |
| Multifect xylanase | 0.15 | 26.6 | 22969 | 26.5 | 454 | n.a. | n.a. |
| Pulpzyme HC | 17.9 | 12.9 | 1343 | 118.2 | 6961 | n.a. | n.a. |
| F-1663/S | 0.36 | 7.6 | 5.0 | <0.01 | 71.6 | 1.1 | ~0.02 |
| F-1663/WS | 0.36 | 5.2 | 39.5 | ~0.05 | 72.7 | 0.98 | ~0.02 |
| F-1663/B | 0.37 | 4.8 | 40.1 | ~0.03 | 69.6 | 0.88 | ~0.02 |
FPA: filter paper activity; n.d.: not detectable.; n.a.: not analyzed.
F-1663/S, WS, B: the crude enzyme supernatants of Trichoderma atroviride TUB F-1663 produced on steam-pretreated spruce, wheat straw and sugarcane bagasse.
The activities presented are the mean values of at least two separate measurements. The standard deviations are <10%, and mainly 1–4% for FPA, <10% for β-glucosidase, 1–5% for xylanase and β-xylosidase, mostly around 10% for endoglucanase, and 5–10% for mannanase and β-mannosidase measurement.