Figure 3.

Histological and immunohistochemical findings in ABV4-inoculated cockatiels. A) Myenteric ganglioneuritis (arrow) in the crop of cockatiel 2 (hematoxyline and eosin [H&E] staining); B) Myenteric ganglioneuritis (arrow) in the ventriculus of cockatiel 3 (H&E staining). C) Positive IHC staining for ABVN associated with focal gliosis (arrow) in the cerebrum of cockatiel 1. Here, the staining appears to be mainly extracellular. D) For negative control, a section parallel to that in C was stained, using pre-immune rabbit serum instead of the anti-ABVN antibody. E) Widespread positive IHC staining for ABVN of neurons and glial cells of the cerebrum of cockatiel 2. At greater magnification, staining of a large neuron and its dendrites is shown (inset). F) For negative control, a section parallel to that in E was stained, using pre-immune rabbit serum instead of the anti-ABVN antibody.