Table 1.
Library | Sequence direction | Number of clones sequenced | Chromatographs checked (EST number) | Sequence quality checked (EST number) | Average length (bp) | Number of contigs b | Number of singletons | ||
Good quality | Poor quality a | Good quality | Poor quality | ||||||
Plasmid | 3'-end | 1,152 | 764 | 388 | 722 | 42 | 441 | ||
Uni-ZAP RX | 5'-end | 13,848 | 12,302 | 1,546 | 11,797 | 505 | 674 | ||
Total | -- | 15,000 | 13,066 | 1,934 | 12,519 | 547 | 656 | 1,157 | 1,738 |
a The poor quality sequences were discarded and were not included in the analysis.
b The numbers of contigs and singletons were based on the analysis of all the ESTs sequenced from the two libraries.