Table 3.
List of genes potentially involved in Bt toxicity and resistance as identified by EST analysis from the guts of the European corn borer larvae
| European corn borer | Silkwormb | |||||||
| EST ID | Matches | Organisma | % Identities | E-value | Matches | % Identities | E-value | |
| Trypsin-like serine proteases | ||||||||
| 1 | Contig [0038] | AAR98918.1 | Ostrinia nubilalis | 254/256 (99%) | 3e-148 | AAB26023.1 | 144/233 (61%) | 2e-78 |
| 2 | Contig [0157] | ACB54937.1 | Helicoverpa armigera | 46/97 (47%) | 8e-18 | No match | -- | -- |
| 3 | Contig [0111] | ABF47507.1 | Ostrinia furnacalis | 248/257 (96%) | 5e-135 | AAB26023.1 | 136/231 (58%) | 2e-64 |
| 4 | Contig [0291] | AAX62039.1 | Ostrinia nubilalis | 257/258 (99%) | 5e-137 | AAB26023.1 | 100/240 (41%) | 1e-43 |
| 5 | Contig [0486] | ABU98624.1 | Helicoverpa armigera | 148/241 (61%) | 1e-72 | AAB26023.1 | 109/235 (46%) | 4e-48 |
| 6 | Contig [0754] | AAX62034.1 | Ostrinia nubilalis | 154/266 (57%) | 7e-69 | AAB26023.1 | 128/244 (52%) | 3e-56 |
| 7 | Contig [0622] | ABU98624.1 | Helicoverpa armigera | 145/249 (58%) | 4e-82 | AAB26023.1 | 101/238 (42%) | 3e-50 |
| 8 | Contig [0907] | ABU98619.1 | Helicoverpa armigera | 89/206 (43%) | 6e-47 | No match | -- | -- |
| 9 | Contig [1007] | AAR98918.1 | Ostrinia nubilalis | 218/252 (86%) | 1e-120 | AAB26023.1 | 119/229 (51%) | 1e-57 |
| 10 | Contig [1400] | ABU98619.1 | Helicoverpa armigera | 56/199 (28%) | 2e-21 | No match | -- | -- |
| 11 | Contig [1615] | ABF47507.1 | Ostrinia furnacalis | 101/189 (53%) | 1e-64 | AAB26023.1 | 95/183 (51%) | 5e-50 |
| 12 | Contig [3395] | AAX62032.1 | Ostrinia nubilalis | 129/209 (61%) | 3e-72 | AAB26023.1 | 117/208 (56%) | 7e-59 |
| 13 | Contig [4300] | AAX62035.1 | Ostrinia nubilalis | 79/84 (94%) | 8e-43 | AAB26023.1 | 43/72 (59%) | 5e-16 |
| 14 | Contig [4291] | AAX62032.1 | Ostrinia nubilalis | 181/236 (76%) | 2e-105 | AAB26023.1 | 138/233 (59%) | 7e-75 |
| 15 | ECB-30_C08 | AAX62036.1 | Ostrinia nubilalis | 36/45 (80%) | 1e-13 | No match | -- | -- |
| 16 | ECB-17_C09 | ABC87051.1 | Ostrinia furnacalis | 65/98 (66%) | 5e-30 | AAB26023.1 | 48/97 (49%) | 4e-19 |
| 17 | ECB-C-18_B11 | AAR98920.2 | Ostrinia nubilalis | 198/204 (97%) | 5e-114 | No match | -- | -- |
| 18 | ECB-V-26_H09 | ABC87051.1 | Ostrinia furnacalis | 35/50 (70%) | 6e-12 | NP_001040350 | 24/48 (50%) | 3e-04 |
| Chymorypsin-like serine proteases | ||||||||
| 1 | Contig [0026] | AAX62029.1 | Ostrinia nubilalis | 258/261 (98%) | 5e-149 | NP_001036903.1 | 163/259 (62%) | 1e-86 |
| 2 | Contig [0058] | AAX62029.1 | Ostrinia nubilalis | 228/261 (87%) | 3e-120 | No match | -- | -- |
| 3 | Contig [0120] | AAF71515.1 | Agrotis ipsilon | 174/287 (60%) | 2e-84 | NP_001040430.1 | 109/244 (44%) | 2e-47 |
| 4 | Contig [0141] | AAX62028.1 | Ostrinia nubilalis | 197/262 (75%) | 1e-101 | NP_001040430.1 | 130/261 (49%) | 2e-52 |
| 5 | Contig [0187] | AAX62026.1 | Ostrinia nubilalis | 193/202 (95%) | 4e-97 | No match | -- | -- |
| 6 | Contig [0299] | AAX62029.1 | Ostrinia nubilalis | 228/261 (87%) | 6e-120 | NP_001036903.1 | 166/259 (64%) | 7e-82 |
| 7 | Contig [0379] | AAX62030.1 | Ostrinia nubilalis | 111/242 (45%) | 1e-55 | NP_001036903.1 | 109/236 (46%) | 8e-55 |
| 8 | Contig [0426] | AAX62026.1 | Ostrinia nubilalis | 282/289 (97%) | 1e-129 | No match | -- | -- |
| 9 | Contig [0560] | NP_001040430.1 | Ostrinia nubilalis | 128/232 (55%) | 1e-63 | NP_001040430.1 | 171/272 (62%) | 4e-93 |
| 10 | Contig [0806] | AAX62029.1 | Ostrinia nubilalis | 202/208 (97%) | 6e-137 | No match | -- | -- |
| 11 | Contig [1061] | CAL92020.1 | Manduca sexta | 169/281 (60%) | 1e-87 | No match | -- | -- |
| 12 | Contig [1478] | NP_001040430.1 | Bombyx mori | 152/260 (58%) | 5e-84 | NP_001040430.1 | 152/260 (58%) | 4e-84 |
| 13 | Contig [2079] | AAL93243.1 | Aedes aegypti | 85/242 (35%) | 7e-40 | No match | -- | -- |
| 14 | Contig [2569] | AAF71518.1 | Helicoverpa zea | 119/240 (49%) | 7e-49 | NP_001040430.1 | 87/212 (41%) | 1e-30 |
| 15 | Contig [4479] | AAC36150.1 | Plodia interpunctella | 140/263 (53%) | 3e-77 | NP_001036826.1 | 117/270 (43%) | 3e-51 |
| 16 | Contig [4699] | AAX62029.1 | Ostrinia nubilalis | 195/261 (74%) | 1e-102 | NP_001036826.1 | 144/251 (57%) | 9e-68 |
| 17 | ECB-23_F02 | CAM84318.1 | Manduca sexta | 88/209 (42%) | 3e-36 | No match | -- | -- |
| 18 | ECB-V-25_E02 | AAX62031.1 | Ostrinia nubilalis | 32/32 (100%) | 2e-11 | NP_001040430.1 | 99/203 (48%) | 6e-42 |
| Aminopeptidases | ||||||||
| 1 | Contig [0722] | AAP37951.1 | Helicoverpa armigera | 72/193 (37%) | 9e-29 | BAA33715.1 | 60/160(37%) | 2e-21 |
| 2 | Contig [1364] | ABL01481.1 | Ostrinia nubilalis | 413/421 (98%) | 0.0 | NP_001037013.1 | 273/422 (64%) | 2e-154 |
| 3 | Contig [1716] | XP_560264.3 | Anopheles gambiae | 132/354 (37%) | 3e-59 | No match | -- | -- |
| 4 | Contig [1907] | ACB87202.1 | Ostrinia furnacalis | 370/374 (98%) | 0.0 | BAA33715.1 | 240/368 (65%) | 8e-135 |
| 5 | Contig [4362] | AAQ57405.1 | Helicoverpa armigera | 102/263 (38%) | 2e-48 | NP_001037013.1 | 89/266 (33%) | 1e-42 |
| 6 | Contig [4298] | ACB47287.1 | Ostrinia furnacalis | 291/297 (97%) | 3e-167 | NP_001036834.1 | 213/297 (71%) | 6e-118 |
| 7 | Contig [4992] | AAP37951.1 | Helicoverpa armigera | 168/246 (68%) | 7e-99 | BAA33715.1 | 85/238 (35%) | 6e-38 |
| 8 | Contig [4529] | ABV01346.1 | Ostrinia furnacalis | 342/356 (96%) | 0.0 | NP_001104835.1 | 226/350 (64%) | 4e-125 |
| 9 | ECB-G02 | AAK85539.1 | Helicoverpa armigera | 196/262 (74%) | 2e-114 | No match | -- | -- |
| 10 | ECB-D07 | ABQ51393.1 | Ostrinia furnacalis | 171/22 (75%) | 1e-99 | NP_001104835.1 | 159/225 (70%) | 2e-95 |
| 11 | ECB-D12 | ABV01346.1 | Ostrinia furnacalis | 200/278 (71%) | 1e-112 | NP_001104835.1 | 155/275 (56%) | 5e-80 |
| 12 | ECB-C06 | ABL01481.1 | Ostrinia nubilalis | 40/40 (100%) | 9e-16 | NP_001037013.1 | 23/39 (58%) | 7e-07 |
| 13 | ECB-F04 | AAP37951.1 | Helicoverpa armigera | 117/208 (56%) | 2e-61 | BAA32475.1 | 67/190 (35%) | 2e-24 |
| Alkaline phosphatases | ||||||||
| 1 | Contig [5091] | NP_001037536.2 | Bombyx mori | 101/172 (58%) | 3e-53 | NP_001037536.2 | 101/172 (58%) | 3e-53 |
| 2 | Contig [2328] | BAF62124.1 | Bombyx mandarina | 176/260 (67%) | 8e-103 | NP_001037536.2 | 177/260 (68%) | 2e-102 |
| Cadherin-like protein | ||||||||
| 1 | ECB-B09 | ABS59299.1 | Ostrinia furnacalis | 242/244 (99%) | 7e-135 | BAA99405.1 | 155/247 (62%) | 1e-81 |
a Sequence with highest score in BLASTX search
b Match of ECB ESTs with silkworm sequence using BLASTX