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. Author manuscript; available in PMC: 2009 Jul 29.
Published in final edited form as: Cell Signal. 2005 Jun 14;18(3):318–327. doi: 10.1016/j.cellsig.2005.05.014

Fig. 6.

Fig. 6

Effect of α2-ARm on ERK1/2 activation by α2A-AR and α2B-AR. HEK293 cells transfected with 4 µg of α2B-AR (A), or α2A-AR (B) together with 8 µg of pEGFP-N1 vector (control, squares) or α2B-ARm-GFP (triangles) were stimulated with increasing concentrations of UK14304 (0.01 to 10 µM) for 5 min. ERK1/2 activation was determined by Western blot analysis using phospho-specific ERK1/2 antibodies. Left panel-representative blots of ERK1/2 activation (upper panel) and total ERK1/2 expression (lower panel); Right panel-quantitative data expressed as percent of the ERK1/2 activation obtained in cells transfected with individual receptors and stimulated with 1 µM UK14304. Similar results were obtained in four separate experiments.