Fig. 3.

TβRIII negatively regulates NFκB signaling via β-arrestin2. MCF10A (A) and MDA-MB-231 (B) cells were infected with adenovirus expressing GFP, TβRIII or TβRIII-T841A and grown for 48 h. Cells were then treated with TGF-β1, 200 pM for 1 h, harvested and subjected to western blotting with the indicated antibodies. (C) MDA-MB-231 cells were transfected with siRNA against β-arrestin2 for 2 days and treated with TGF-β1 (200 pM, 1 h), after which cells were harvested for western blotting. The results shown are representative of at least three independent experiments.