Figure 3.

Bragg peaks from GIXD of 600 μg Survanta spread onto a saline buffer subphase containing 2 mg/mL albumin. The first two panels show different regions of the film at the same surface pressure. The top panel shows no Bragg peaks in region 1, consistent with an albumin-covered interface. The second panel shows that in the adjacent region (region 2), Bragg peaks reveal the presence of Survanta. The third panel shows that the Survanta lattice compacts with increasing surface pressure; however, surface pressures higher than 28 mN/m could be sustained due to the presence of albumin. The last panel shows GIXD at 20 mN/m after two additional cycles; the Survanta peaks are no longer present.