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(A) Force as a function of time recorded as the DNA molecule is left to equilibrate after being pulled to different extensions at 3 μm/s pulling speed in the presence of 100 nM YOYO. Curves a–e represent constant extension of 21.5, 22.5, 23.5, 24.5, and 25.5 μm, respectively. (B) The number of YOYO molecules bound to DNA as function of time during force relaxation calculated using Eq. 2.
