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Characteristic time constant as function of the extension, obtained by fitting the force relaxation curves in Fig. 4A with a single-exponential function
| Extension (μm) | 21.5 | 22.5 | 23.5 | 24.5 | 25.5 |
| Time constant τFR (s) | 0.23 | 0.33 | 0.64 | 1.13 | 2.54 |
The DNA-YOYO complex was pulled to the present extension at 3 μm/s pulling speed. Fitted curves are presented in Fig. S2.
