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. 2009 Aug 5;97(3):866–874. doi: 10.1016/j.bpj.2009.04.056

Figure 2.

Figure 2

CW-EPR spectra of ferric HbTb at different pH values. Experimental conditions as in Fig. 1. Spectra in the left panel are replotted on a ×10 intensity scale on the right, showing the low-spin signal region. The g = 5.88 regions of the high-spin signals are shown in the inset: pH 4.5 (black), pH 6.0 (red), pH 7.6 (green), pH 9.0 (blue), and pH 10.0 (pink). The signal intensities are normalized against that of the pH 7.6 sample. The increase in line width in the down-field region of the signal indicates an increase in rhombicity.