Fig. 8.

SDS–PAGE analysis and in-gel kinase assay of p46MAPK from the Hiload 16/60 Superdex 75pg column. Protein from the Hiload 16/60 Superdex 75pg column was separated on a 12% SDS–polyacrylamide gel. Lane 1, molecular mass markers in kilodaltons; lane 2, SDS–PAGE was stained with Coomassie blue; lane 3, SDS–PAGE was stained with silver; lane 4, lane 3 analysed by Quantity One software; lane 5, in-gel kinases assay of purified protein kinase. Protein from Hiload 16/60 Superdex 75pg column was loaded onto a 12% SDS–polyacrylamide gel embedded with MBP.