Table 1.

Partial purification of p46MAPK from maize leaves

Step of purification	Protein (mg)	Total activity (pmol min−1)	Specific activity (pmol min−1 mg−1)	Fold of purification	Recovery (%)
Homogenatea	3740	7106	2	1	100.0
30% (NH4)2SO4	258	4489	17	9	63.2
100 000 g	171	3454	20	10	48.6
Q-Sepharose FF	11.56	983	85	43	13.8
Phenyl-Sepharose FF (HS)	1.73	415	240	120	5.8
Resource Q	0.368	307	834	417	4.3
Mono Q™ 5/50 GLb	0.138	249	1 804	902	3.5
Poly-L-lysine-agaroseb	0.021	189	9 005	4 502	2.7
Hiload 16/60Superdex 75pgb	0.004	82	20 420	10 210	1.1

^aThe starting homogenate was prepared from 1000 g of maize leaves treated with 100 μM ABA for 2 h. MBP was used as substrate in the kinase assays. Total protein amount was measured according to Bradford (1976).

^bThe amount of protein was estimated by comparison with known bovine serum albumin on a Coomassie-stained SDS–polyacrylamide gel.