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. 2009 Apr 29;587(Pt 12):2903–2918. doi: 10.1113/jphysiol.2009.170662

Figure 1. Overexpression of STIM1 and Orai1 in H4IIE cells.

Figure 1

A, examples of development of the Orai1/STIM1 mediated current. Each point represents the amplitude of the current at −118 mV measured from the responses to voltage ramps ranging from −138 mV to 102 mV and applied every 2 s. B, examples of the I–V plots obtained from cells expressing both STIM1 and Orai1. C, examples of the currents obtained in response to 200 ms steps to −138 mV. Traces were taken 40–60 s after obtaining whole-cell configuration when the amplitude of the current reached steady-state. Note significant variations in current kinetics. Cells were transfected with Orai1/STIM1 in a molar ratio of 1 : 1. Leakage was determined by applying 50 μm 2-APB at the end of the experiment and was corrected for in panels B and C, but not A.