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. 2009 Apr 29;587(Pt 12):2937–2947. doi: 10.1113/jphysiol.2009.172700

Figure 5. The NMDA NR1a (C744A,C798A) mutant shows markedly diminished sensitivity to blue-light potentiation.

Figure 5

A, sample trace showing only a small degree of blue-light potentiation of the response to 10 μm NMDA in the presence of 300 nm C2-NBD 3α5αP in the NR1a (C744A,C798A)-NR2B receptors expressed in HEK 293 cells. B, sample trace showing blue-light potentiation of wild-type NR1a-NR2B NMDA receptors. C, summary bar graph of blue-light potentiation of mutant receptors, wild-type receptors and 50 μm pregnenolone sulfate (Preg sulfate) potentiation of mutant receptors. Error bars represent s.e.m. Baseline response is 100% (dashed line). Potentiated currents from both mutant and wild-type receptors were significantly different from current prior to activation of the light or application of pregnenolone sulfate (paired t test, P < 0.05). Potentiation of mutant receptors was significantly less than potentiation of wild-type receptors (Student's t test, P < 0.05). Recordings were performed with CsMeSO4 internal solution at −60 mV.