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. 2009 Apr 22;96(8):3210–3222. doi: 10.1016/j.bpj.2008.12.3923

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Amount of PCer generated by PSM hydrolysis by SMase. (A) Fraction of PCer formed versus time in (○) 30% and (▿) 40% PSM-containing binary mixtures and in POPC/PSM/Chol ternary mixtures containing (▴) 20 (T1), (▵) 23 (T2), (♦) 26 (T3), (□) 30 (T4), (▪) 33 (T5), (▾) 35 (T6), and (•) 37% (T7) PSM, respectively. (B) Relationship between the fraction of PCer formed and the initial substrate available for hydrolysis in POPC/PSM/Chol ternary mixture at (▾) t = 0.5, (♦) 2, (▵) 5, (▿) 30, and (•) 120 min, respectively. The values are the means of at least three independent experiments. The SD was always <3%.