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. 2009 Apr 22;96(8):3210–3222. doi: 10.1016/j.bpj.2008.12.3923

Figure 6.

Figure 6

PCer-induced alterations in the membrane physical properties of raft mixtures. Fluorescence anisotropy of (A) (▪,□) t-PnA, and (B) (▴,▵) Rho-DOPE and (•,○) NBD-DPPE in the absence of SMase (solid symbols) and 2 h after SMase addition (open symbols). The mean fluorescence lifetimes of (C) t-PnA and (D) NBD-DPPE are shown (the symbols are the same as in A and B, respectively). The values are the means of at least three independent experiments. The SD was always lower than (A and B) 0.01, (C) 2 ns for t-PnA, and (D) 0.5 ns for NBD-DPPE.