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. 2009 Apr 22;96(8):3390–3398. doi: 10.1016/j.bpj.2009.01.032

Figure 2.

Figure 2

(A) Ultramicroscopy image from 0.2 mm-thick stack from RN of 396-day-old wt mock mouse. The image quality is comparable to the confocal imaging (B). (B) Confocal image of the RN, 70-day-old wt mock mouse. (C) 3D reconstruction of 0.2 mm-thick ultramicroscopy RN stack, the same specimen as in A. The ultramicroscopy enables 3D reconstruction of superior quality and containing lower background as compared to the confocal one (D). (D) 3D reconstruction of six serial confocal stacks from RN. Because of cryosectioning, the reconstruction contains high background. (E) 3D reconstruction of ultramicroscopy stacks from prion-challenged Tga20 mouse. The side of the RN contralateral to prion inoculation contains reduced amount of REx-positive cells (arrows). Scale bars, 100 μm.