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SEM images showing the effect of topographic micropatterning on SMCs on micropatterned and nonpatterned PDMS membranes (24-h culture). (A) Cross-sectional view of 10-μm-wide microgrooves. (B) Top view of microgrooves. (C and D) Phalloidin staining of actin stress fibers on (C) nonpatterned and (D) micropatterned surfaces (80–90% confluency). (E and F) ToPro nuclear staining on (E) nonpatterned and (F) micropatterned surfaces. (C–F, scale bar = 50 μm.)
