Figure 2.

Micropatterning effects on cell shape, nucleus shape, cell spreading area, and cell proliferation rate. SMCs were cultured on nonpatterned and micropatterned (10-μm-wide grooves) surfaces (24-h culture, unless otherwise specified). (A) CSI determined from fluorescent images of SMCs (50 cells per group; Fig. S3). (B) NSI determined from nucleus fluorescent staining images of SMCs (50 cells per group). (C) Spreading area of SMCs (50 cells per group). (D) SMC proliferation. After a 48-h culture, SMCs were incubated with BrdU for 2 h and then fixed and double-stained for BrdU and nuclei. The percentage of BrdU-positive cells (cells in S phase) was calculated using >500 cells per group. Bars represent mean ± SD. Data were extracted from at least three experiments. The asterisk indicates the statistically significant difference (p < 0.05) between specified groups.