Micropatterning effects on gene expression and role of NOR-1 in SMC proliferation. (A) Gene expression quantified by qRT-PCR experiments (n = 3, 24-h culture). (B) Immunoblotting analysis of NOR-1, α-actin, and tubulin expression (48-h culture). (C) NOR-1 knock-down effect on NOR-1 expression and SMC proliferation (>300 cells per group, three independent experiments, 48-h culture). Bars show mean ± SD. The asterisk indicates the statistically significant difference (p < 0.05) between specified groups.