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. 2009 Apr 22;96(8):3423–3432. doi: 10.1016/j.bpj.2008.11.074

Figure 5.

Figure 5

Cell shape effects on SMC proliferation. (A) Actin (green) and nuclear (red) staining in an SMC micropatterned in a circular shape (24-h culture). (B) Actin (green) and nuclear (red) staining in an SMC micropatterned in a spindle-like shape (24-h culture). (C) NSI determined from nuclear fluorescent staining images of SMCs cultured on nonpatterned and micropatterned surfaces (>15 cells per group, 24-h culture). (D) BrdU incorporation for cases I–IV (24-h culture; Fig. 4A). Bars represent mean ± SD. The asterisk indicates the statistically significant difference (p < 0.05) compared to all other groups (>200 cells per group, three independent experiments).