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. 2009 Apr 22;96(8):3423–3432. doi: 10.1016/j.bpj.2008.11.074

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Cell shape and spreading area effects on nucleus morphology (24-h culture). SMCs of specific shape and spreading area were subjected to fluorescence staining for actin filaments (green) and nucleus (blue). (A) Confocal microscopy images of actin filaments and 3D reconstruction of the nucleus shape. (B) Calculated CSI and cell spreading area (3–10 cells per group). (C) Calculated NSI and nucleus volume (3–10 cells per group). The asterisk indicates the statistically significant difference (p < 0.05) between specified groups or compared to all other groups.