Potential periodicity, δ
|
|
2π/26 |
See (7,17). |
Rotor drag coefficient, ζR
|
|
2 × 10−3 pN·nm·s/rad2
|
Estimated |
Bead diffusion constant, DL
|
|
0.01–100 rad2/s |
Calculated from Stokes' Law |
Stator diffusion constant, Ds
|
|
500 rad2/s |
Estimated |
Load-rotor linkage spring constant, κ
|
|
400–500 pN·nm/rad2
|
Estimated from experimental measurements (49) |
Saw-tooth potential height, U
|
|
10 kBT
|
Ad hoc |
Ratio of the two potential branches, Lleft/Lright
|
|
1/9 |
Potential bumps |
Height |
15 kBT
|
|
Width |
0.2δ
|
|
Centers |
0.1δ (State1) |
|
0.6δ (State 2) |
Transition windows |
α1, α2
|
0.1δ, 0.6δ
|
Fitting data |
β1β2
|
0.58δ, (0.58 + 0.5)δ
|
Binding site pK value |
H+ motor |
pKa= 7.3 |
Estimated (using the middle value of external and internal concentrations) |
Chimera motor |
pKa= 31.6 mM |
Binding rate prefactors (two ions) k0
|
H+ motor |
1.0 × 1020 s−1
|
Fit experimental torque-speed curve |
Chimera motor |
1.0 × 108 s−1 (or 6.0 × 107 s−1 with a uniform window) |
E. coli BFM living condition |
pHperiplasm
|
7.0 |
Experimental values |
pHcytoplasm
|
7.6 |
Vmembrane
|
120 mV |
Chimera BFM living condition |
[Na]periplasm
|
85 mM |
[Na]cytoplasm
|
12 mM |
Vmembrane
|
140 mV |