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Measurement of aromatase activity in the uterus during early pregnancy
| Tissue | [3H]water released, fmol/mg net wt/24 h | Estradiol, pg/mL of uterine extract |
|---|---|---|
| Uterus (d 6) | 24 ± 3.2 | 12.12 ± 3.22 |
| Uterus (d 6 + AI) | 8.7 ± 1.5 | Undetectable |
| Ovary | 45 ± 5.4 | Not analyzed |
The tissue homogenates were incubated with [1β-3H]androstenedione for 6 h at 37 °C to estimate the water release per milligram of tissue. The intrauterine levels of estrogen were analyzed by radioimmunoassay.
