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Binding of host–guest fusion peptides to lipid bilayers composed of POPC:POPG (4:1, mol/mol) at pH 7.4 or pH 5.0. The concentrations of the NBD-labeled peptides were 0.05 or 0.1 μM. The binding isotherms were obtained at 25°C by plotting Xb (moles of bound peptide per moles of accessible lipid) vs. the equilibrium concentration of free peptide in solution, Cf (see text).
