Figure 1.

Surfactant protein D (SP-D)-deficient mice have increased susceptibility to intratracheal bleomycin. SP-D–deficient mice and C57BL/6 control mice (Jackson Research Laboratories) 8 to 9 wk of age received either saline (n = 5 for each strain), 1 U/kg intratracheal bleomycin (n = 15 for each strain), or 2 U/kg intratracheal bleomycin (n = 15 for each strain) as described in METHODS. Survival for each group was recorded throughout a 21-d observation period and plotted as Kaplan-Meier survival analysis. Comparison of mortality at Day 14 revealed enhanced susceptibility at both 1 U/kg and 2 U/kg bleomycin in the SP-D (−/−) groups (p < 0.05 versus corresponding control groups). Data are representative of three separate experiments.