Figure 9.

3-nitrotyrosine/NO ratio and macrophage size are increased after intratracheal bleomycin. BAL and lung tissue from SP-D OE and littermate control mice were harvested 8 d after administration of 3 U/kg bleomycin. (A) BAL NO levels and tissue 3-nitrotyrosine (NO₂Y) content were measured as described in METHODS. Data, expressed as the ratio of NO₂Y to NO, demonstrate an increase in NO₂Y/NO in ITB-treated WT mice compared with ITB-treated SP-D OE mice. *p < 0.05. (B) Manual counting and determination of macrophage size were performed on cytospins (n = 30–50 macrophages/cytospin sample × 6–8 cytospins) in bleomycin-treated SP-D OE (square) and littermate (triangle) mice. NO₂Y/NO (y axis) plotted as a function of the percentage of macrophages greater that 25 μ in diameter (x axis) positively correlated with macrophages size (r = 0.459, n = 6–8).