TABLE 6.
COMPARISON OF mRNA ALTERATIONS IN THE DIAPHRAGM AFTER 6 AND 18 h MECHANICAL VENTILATION AND LOCOMOTOR SKELETAL MUSCLE (SOLEUS) AFTER 12 h HIND-LIMB UNLOADING
| Diaphragm
|
Locomotor Muscle
|
||||||
|---|---|---|---|---|---|---|---|
| GenBank Accession
|
MV6/Control
|
MV18/Control
|
Inactivity/Control
|
||||
| p Value* | Fold† | p Value* | Fold† | p Value* | Fold† | Description | |
| J03969 | 3.23E-01 | 0.6 | 1.02E-04 | 1.8 | 5.6E-06 | 2.0 | Nucleophosmin 1 |
| S74351 | 2.63E-05 | −7.2 | 2.06E-03 | −5.5 | 5.6E-07 | −2.3 | Protein tyrosine phosphatase, nonreceptor type 16 |
| AI639233 | 5.54E-01 | 0.6 | 6.98E-05 | 2.0 | Decorin | ||
| Z12298 | 6.7E-07 | 6.1 | Decorin | ||||
| X59859 | 4.0E-06 | 3.2 | Decorin | ||||
| X59859 | 3.2E-05 | 2.7 | Decorin | ||||
| L23148 | 1.04E-04 | −2.5 | 2.15E-03 | −2.6 | 8.9E-07 | −2.4 | Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation) |
| U02553 | 5.73E-04 | −3.3 | 6.87E-03 | −3.1 | 4.5E-06 | −2.5 | Protein tyrosine phosphatase, nonreceptor type 16 |
| L32591 | 1.56E-03 | 6.7 | 1.94E-05 | 13.2 | < 1E-08 | 2.4 | Growth arrest and DNA damage–inducible 45α |
| L32591 | 1.32E-04 | 8.3 | 4.62E-08 | 16.7 | 2.4E-06 | 2.0 | Growth arrest and DNA damage–inducible 45α |
| AF039583 | 3.12E-04 | −5.7 | 1.07E-04 | −18.8 | 1.3E-06 | −2.8 | Decay-accelerating factor |
| M32167 | 9.3E-06 | −2.0 | VEGF | ||||
| L20913 | 1.04E-01 | −0.9 | 3.34E-04 | −1.6 | VEGF | ||
| S85184 | 1.47E-01 | 2.0 | 3.55E-08 | 4.2 | 4.4E-07 | 4.7 | Cathepsin L proenzyme |
| S85184 | 1.03E-01 | 2.8 | 4.25E-07 | 6.5 | 1.5E-05 | 2.7 | Cathepsin L proenzyme |
| U77829 | 2.24E-02 | 2.0 | 1.97E-05 | 3.9 | 4.2E-04 | 2.5 | Rattus norvegicus gas-5 growth arrest homolog nontranslated mRNA sequence |
| U77829 | 3.51E-02 | 2.4 | 3.36E-05 | 5.3 | Rattus norvegicus gas-5 growth arrest homolog nontranslated mRNA sequence | ||
| D42137 | 2.83E-02 | −1.1 | 9.35E-04 | −1.7 | 1.3E-05 | −2.1 | Annexin V |
| U50736 | 6.65E-02 | 16.8 | 1.91E-06 | 56.5 | 2.2E-07 | −2.7 | Ankyrin-like repeat protein |
| AI169370 | 2.89E-01 | 0.9 | 9.12E-04 | 2.2 | α-Tubulin | ||
| V01227 | 3.3E-07 | −2.4 | α-Tubulin | ||||
| M86564 | 9.04E-01 | −0.4 | 3.99E-04 | 1.9 | 1.9E-06 | −1.6 | α-Prothymosin |
| X15580 | 9.06E-01 | −1.1 | 2.27E-04 | −5.3 | 2.6E-06 | 3.4 | 6-phosphofructo-2 kinase/fructose 2,6-biphosphatase 1 |
| S68135 | 2.35E-02 | 2.9 | 7.31E-04 | 10.4 | 3.3E-05 | −2.1 | GLUT1 |
| S63233 | 4.23E-03 | 1.4 | 2.13E-04 | 2.3 | 1.6E-06 | −2.1 | Phosphoglycerate mutase type B |
| L19998 | 3.20E-01 | −0.9 | 6.12E-04 | −2.8 | 3.3E-07 | 2.8 | Sulfotransferase family 1A, phenol-preferring, member 1 |
| L19998 | 1.9E-06 | 2.5 | Sulfotransferase family 1A, phenol-preferring, member 1 | ||||
| U12187 | 1.02E-04 | 12.8 | 1.52E-05 | 17.1 | 3.3E-07 | −8.4 | ras-related protein |
Definition of abbreviation: VEGF = vascular endothelial growth factor.
Note that the locomotor skeletal muscle mRNA data are from a previous study (21) using similar methods to the current study. Genes that responded in a similar fashion to mechanical ventilation and hind-limb unloading are above the horizontal line and those that responded antithetically are below the line.
*
p Value calculated by Wilcoxon's signed rank test.
†
Fold indicates the fold change from control values.