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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Cancer Res. 2009 May 26;69(12):4954–4958. doi: 10.1158/0008-5472.CAN-09-0584

Figure 2.

Figure 2

Transcriptional repression of survivin by PTEN. A, the indicated cell types were transfected with pEGFP or increasing concentrations of PTEN cDNA, and analyzed by Western blotting. *,nonspecific. B, HCT116 cells expressing pEGFP or PTEN cDNA were transfected with a survivin promoter luciferase construct (pLuc-649), and analyzed for luciferase activity. **, P = 0.0048. Columns, mean of replicates of a representative experiment of at least two independent determinations; bars, SE. C, HCT116 cells were transfected with ms-830-GFP, treated with vehicle or LY294002 (30 µmol/L), and analyzed by fluorescence microscopy. Right, Western blotting. D, MCF-7 cells were transfected with pcDNA or PI3 kinase p85Δ DN mutant and analyzed by Western blotting.