Transcriptional repression of survivin by PTEN. A, the indicated cell types were transfected with pEGFP or increasing concentrations of PTEN cDNA, and analyzed by Western blotting. *,nonspecific. B, HCT116 cells expressing pEGFP or PTEN cDNA were transfected with a survivin promoter luciferase construct (pLuc-649), and analyzed for luciferase activity. **, P = 0.0048. Columns, mean of replicates of a representative experiment of at least two independent determinations; bars, SE. C, HCT116 cells were transfected with ms-830-GFP, treated with vehicle or LY294002 (30 µmol/L), and analyzed by fluorescence microscopy. Right, Western blotting. D, MCF-7 cells were transfected with pcDNA or PI3 kinase p85Δ DN mutant and analyzed by Western blotting.