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Histochemical staining for hyaluronan (HA) in wild-type and Has3−/− mice. Histochemical staining of total HA in lungs of (A) the control group of wild-type, (B) the high VT group of wild-type, (C) the control group of Has3−/−, and (D) the high VT group of Has3−/− mice. There was an increase of total HA accumulation in the large airways and parenchyma of lung in wild-type mice with high VT ventilation.
