Figure 6.
High VT–ventilation-induced low-molecular-weight (LMW) HA in lung (n = 5/group). HA from lung tissues was run on Sepharose CL-4B (150 × 2 cm; total volume [Vt] 120 ml, void volume [Vo] 30-ml column) for chromatography. Three standard HA: HMW HA (1,600 kD) had a relative elution volume (Kav) of 0.17, LMW HA (370 kD) had a Kav of 0.33, and LMW HA (178 kD) had a Kav of 0.8. The HA in wild-type mice ventilated with high VT (closed squares) had a Kav of 0.17 and a Kav from 0.55 to 0.77. The HA of wild-type control (closed diamonds), wild-type low VT (closed triangles), Has3−/− control (open diamonds), Has3−/− low VT (open triangles), and Has3−/− high VT (open squares) had a Kav of 0.17.