Figure 10.

Immunolocalization of nitrotyrosine in response to HVT. Colocalization of nitrotyrosine (red signal, a–f), the endothelial cell marker CD34 (green signal; a, c, and e), and type II epithelial cell marker surfactant protein C (SPC; green signal; b, d, and f) in C57BL/6J exposed to HVT (a and b), LPS (c and d), and in control, untreated mouse lungs (e and f). Note the preferential colocalization of nitrotyrosine and CD34 in lungs from mice exposed to HVT (yellow signal, a) as compared with expression of nitrotyrosine in type II, SPC-positive cells (b). There was more nitrotyrosine accumulation in lungs of mice exposed to HVT (a and b) as compared with room-air control animals (e–f). Lungs of LPS-treated mice displayed intense nitrotyrosine expression, mostly present in septal space and presumably septal neutrophils (c–d).