Skip to main content
. 2005 Jun 3;172(4):470–479. doi: 10.1164/rccm.200411-1547OC

Figure 3.

Figure 3.

Figure 3.

(A) Induction of iNOS protein by MV is dependent on VT. Upper panel: Immunoblots were prepared from extracts of lungs of C57BL/6J (WT) and iNOS−/− mice breathing spontaneously (controls) or exposed to MV with LVT (6 ml/kg) and HVT (20 ml/kg) as described in METHODS. Lower panel: Densitometric assessment of iNOS/actin protein expression (n ⩾ 3 for each condition). iNOS protein expression was undetectable in control iNOS−/− or HVT-exposed iNOS−/− mice. iNOS protein expression was minimal in the WT sham group and increased significantly (3.5-fold) in response to HVT for 2 hours. *p < 0.05 versus sham. (B) Induction of iNOS protein by HVT is time-dependent. C57BL/6J mice were exposed to HVT MV and killed at various time points (three animals/time point) before iNOS immunoblotting. *p < 0.05 versus Time 0. Upper panel: iNOS and actin immunoblots. Lower panel: Densitometric assessment of iNOS/actin protein expression (n = 3 for each condition).