TABLE 2.
Nitrated proteins in lung cancer
| Proteins | pI | Mol. Wt. (kD) | Accession No. | Function |
|---|---|---|---|---|
| Antioxidant proteins | ||||
| Mn superoxide dismutase | 8.4 | 24.9 | 1070454 | Dismutation of superoxide radicals generating H2O2 |
| Antioxidant protein 2 | 6.0 | 25.1 | 4758638 | H2O2 breakdown and phospholipid turnover |
| Carbonic andydrase I* | 6.5 | 29.1 | 4502517 | Catalyze the reversible hydration of CO2 |
| Selenium binding protein | 5.8 | 53.1 | 16306550 | Participates in intra-golgi protein transport |
| Aldose reductase | 6.56 | 35.85 | 113596 | Reduction of carbonyl-containing compounds |
| Metabolic enzymes | ||||
| Aldolase A* | 8.4 | 39.28 | 229674 | Glycolytic enzyme |
| Triosephosphate isomerase* | 6.4 | 26.7 | 4507645 | Glycolytic enzyme |
| GAPDH | 8.57 | 36.2 | 62503 | Glycolytic enzyme |
| Phosphoglycerate mutase-1 | 6.7 | 28.8 | 4505753 | Glycolytic enzyme |
| Pyruvate kinase | 7.57 | 58.3 | 20178296 | Glycolytic enzyme |
| α-Enolase* | 6.9 | 47.3 | 119339 | Glycolytic enzyme |
| 3-oxoacid CoA transferase | 7.2 | 56.6 | 14290472 | Key enzyme in ketone body metabolism |
| Mit. aldehyde dehydrogenase-2 | 6.6 | 56.4 | 25777732 | Involved in proline degradation pathway |
| Cytosol aminopeptidase | 6.3 | 53.1 | 12643394 | Processing and turnover of intracellular proteins |
| Lipid metabolism | ||||
| Peroxisomal enoyl-CoA hydratase* | 6.6 | 36.3 | 2135896 | Key protein in fatty acid metabolism |
| Apolipoprotein A | 5.6 | 30.7 | 4557321 | Lipid metabolism |
| Apoptosis | ||||
| Annexin I* | 6.6 | 38.9 | 71756 | Regulates activity of Ca2+-phospholipid |
| Annexin III | 5.6 | 36.4 | 4826643 | Regulate cellular growth and signal transduction |
| Annexin VII | 6.3 | 50.4 | 4502111 | Ca2+- and phospholipid-binding protein |
| Structure proteins | ||||
| Vimentin | 4.9 | 53.3 | 13629179 | Structural protein |
| α1-Actin* | 5.2 | 42.4 | 4501883 | Structural protein |
| β-Tubulin* | 4.7 | 48.9 | 2119276 | Structural protein |
| α-Tubulin | 4.9 | 49.9 | 32015 | Structural protein |
| Tubulin α-β dimer | 5.2 | 47.9 | 3745822 | Structural protein |
| Others | ||||
| Transferrin | 6.8 | 77.1 | 4557871 | Plasma protein and carrier for iron |
| Guanine nucleotide binding protein | 7.6 | 35.0 | 1321585 | Activation of signal transduction |
| Fibrinogen γ–α chain* | 5.7 | 49.5 | 71827 | Involved in clot formation |
| β-Fibrinogen | 8.3 | 54.8 | 182430 | Involved in clot formation |
| hnRNPK | 5.4 | 50.9 | 241478 | Pre-mRNA processing, mRNA transport and transcription factor |
Definition of abbreviations: GAPDH = glyceraldehyde 3-phosphate dehydrogenase; hnRNPK = heterogenous nuclear ribonucleoprotein K.
*
Nitrated proteins in tumor and tumor-free tissue.