Fig. 2.

Radial migration of a stellate/basket cell from the top of the IGL through the PCL to the top of the ML, and subsequent change in the direction of migration from radial to tangential at the top of the ML. (A1) Time-lapse series of images showing the migration of a stellate/basket cell from the IGL through the PCL to the top of the ML of P9 mouse cerebellum. Elapsed time after in vitro (in hours) is indicated on the top-right of each photograph. Asterisks and arrows mark the soma and the leading process, respectively. Arrowheads represent the branch of the leading process. Scale bar; 24 µm. (A2-A4) Superimposed image (A4) of a fluorescent image (A2) and a transmitted image (A3) obtained at 32 hours after in vitro in A1. An asterisk marks the soma. A scale bar; 24 µm. (B1–B3) Pseudocolor images represent images of a stellate/basket cell taken every half hour shown in A1. Six images of the cell are superimposed in both B1 and B2, and five images are superimposed in B3. The numbers represent elapsed time after in vitro (in hours). Scale bar; 20 µm. (C1 and C2) The total distance traversed by the cell (C1) shown in A1 and the direction and distance traveled by the cell during each half hour of the testing period (C2) were plotted as a function of elapsed time after in vitro