Fig. 5.

Effect of pimecrolimus on eosinophil survival. Purified eosinophils were preincubated for 30 min without or with varying concentrations of pimecrolimus [n = 11 (IL5) or 7 (sIgA) for normals, n = 7 for atopics]. IL5 (75 pg/ml) or soluble sIgA (10 μg/ml) was then added and cell survival was measured 4 days later. Data are presented as percent survival relative to that induced by stimuli (IL5 or sIgA) alone. In all instances, the mean percent survival in the presence of medium alone (i.e. with no stimulant) was within the range of 17 to 25%. Significant changes in percent survival relative to stimuli alone (i.e. 0 nM pimecrolimus) are indicated by asterisks. ∗ p < 0.05; ∗∗ p < 0.01. Standard errors of the means are indicated by unidirectional error bars.