Table 2.

Lumefantrine susceptibility levels in chloroquine resistance transporter gene (pfcrt)–modified and control lines.

Line	LMF IC50, nmol/La	Recombinant	Parental line	PfCRT haplotype									CQb		ARTM IC50,b nmol/L	MQ IC50,b nmol/L
				72	74	75	76	220	271	326	356	371	IC50, nmol/L	Phenotype
GC03	85.5 ± 7.2	No	…	C	M	N	K	A	Q	N	I	R	29.2 ± 2.0	Susc.	39.4 ± 6.9	52.6 ± 5.4
Dd2	55.2 ± 9.6	No	…	C	I	E	T	S	E	S	T	I	195.1 ± 25.3	Res.	46.3 ± 7.6	46.8 ± 2.0
7G8	27.0 ± 4.1	No	…	S	M	N	T	S	Q	D	L	R	140.4 ± 15.0	Res.	21.6 ± 6.3	9.6 ± 1.3
C2GC03	74.0 ± 7.7	Yes	GC03	C	M	N	K	A	Q	N	I	R	22.9 ± 2.8	Susc.	39.1 ± 7.0	54.7 ± 1.5
C4Dd2	48.7 ± 4.3c	Yes	GC03	C	I	E	T	S	E	S	T	I	147.4 ± 13.3d	Res.	12.6 ± 1.8d	33.1 ± 3.1d
C67G8	46.3 ± 4.6d	Yes	GC03	S	M	N	T	S	Q	D	L	R	126.9 ± 17.2d	Res.	17.2 ± 3.0d	27.3 ± 3.4d
T76K-1Dd2	73.2 ± 10.5d	Yes	Dd2	C	I	E	K	S	E	S	T	I	22.1 ± 2.1d	Susc.	40.4 ± 11.7	27.2 ± 8.0c
C-1Dd2	33.7 ± 5.0	Yes	Dd2	C	I	E	T	S	E	S	T	I	160.7 ± 28.9	Res.	32.9 ± 6.6	36.9 ± 4.2
T76K-17G8	68.3 ± 11.7d	Yes	7G8	C	I	E	K	S	Q	D	L	R	23.9 ± 4.6e	Susc.	13.8 ± 3.2	3.6 ± 0.7
C-17G8	33.6 ± 5.5	Yes	7G8	S	M	N	T	S	Q	D	L	R	108.8 ± 15.9	Res.	7.4 ± 2.1	3.1 ± 0.9

NOTE. ARTM, artemisinin; CQ, chloroquine; LMF, lumefantrine; MQ, mefloquine; Res., resistant; Susc., susceptible.

^aLMF IC₅₀ values are expressed as the mean value (±SE) for pfcrt–modified and control lines. Data were derived from 5–12 independent assays performed in duplicate. (Results from Mann-Whitney U tests compared mutants and recombinant controls).

^bCQ, ARTM, and MQ IC₅₀ values (from at least 3 independent assays performed in duplicate) are expressed as the mean value (±SE) and are reproduced from [19] for the top 6 lines and from [20] for the bottom 4 lines.

^cP < .05.

^dP < .01.

^eP < .001.